Angiotensin-II-Evoked Ca²⁺ Entry in Murine Cardiac Fibroblasts Does Not Depend on TRPC Channels.

ABSTRACT: TRPC proteins form cation conducting channels regulated by different stimuli and are regulators of the cellular calcium homeostasis. TRPC are expressed in cardiac cells including cardiac fibroblasts (CFs) and have been implicated in the development of pathological cardiac remodeling including fibrosis. Using Ca²⁺ imaging and several compound TRPC knockout mouse lines we analyzed the involvement of TRPC proteins for the angiotensin II (AngII)-induced changes in Ca²⁺ homeostasis in CFs isolated from adult mice. Using qPCR we detected transcripts of all Trpc genes in CFs; Trpc1, Trpc3 and Trpc4 being the most abundant ones. We show that the AngII-induced Ca²⁺ entry but also Ca²⁺ release from intracellular stores are criti

SUBMITTER: Camacho Londono JE 

PROVIDER: S-EPMC7072683 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature