Tissue Distribution and Receptor Activation by Somapacitan, a Long Acting Growth Hormone Derivative.

ABSTRACT: Somapacitan is a long-acting, once-weekly, albumin-binding growth hormone (GH) derivative. The reversible albumin-binding properties leads to prolonged circulation half-life. Here, we investigated and compared somapacitan with human GH on downstream receptor signaling in primary hepatocytes and hepatocellular models and using isothermal titration calorimetry to characterize receptor binding of somapacitan in the presence or absence of human serum albumin (HSA). With non-invasive fluorescence imaging we quantitatively visualize and compare the temporal distribution and examine the tissue-specific growth hormone receptor (GHR) activation at distribution sites. We found that signaling kinetics were slightly more rapid and intense for GH compared with somapacitan. Receptor binding isotherms we

SUBMITTER: Petersen M 

PROVIDER: S-EPMC7072805 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature