Detection of MET Alterations Using Cell Free DNA and Circulating Tumor Cells from Cancer Patients.

ABSTRACT: MET alterations may provide a potential biomarker to evaluate patients who will benefit from treatment with MET inhibitors. Therefore, the purpose of the present study is to investigate the utility of a liquid biopsy-based strategy to assess MET alterations in cancer patients. We analyzed MET amplification in circulating free DNA (cfDNA) from 174 patients with cancer and 49 healthy controls and demonstrated the accuracy of the analysis to detect its alteration in patients. Importantly, a significant correlation between cfDNA concentration and MET copy number (CN) in cancer patients (r = 0.57, p <10-10) was determined. Furthermore, we evaluated two approaches to detect the presence of MET on circulating tumor cells (CTCs), using the CellSearch® and Parsortix systems and monitored patients u

SUBMITTER: Mondelo-Macia P 

PROVIDER: S-EPMC7072825 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature