MRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules.

ABSTRACT: Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in dcp2 and xrn1 mutants and in four mutants with constitutive stress granule

SUBMITTER: Kozlova NV 

PROVIDER: S-EPMC7072924 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature