Interactome and F-Actin Interaction Analysis of Dictyostelium discoideum Coronin A.

ABSTRACT: Coronin proteins are evolutionary conserved WD repeat containing proteins that have been proposed to carry out different functions. In Dictyostelium, the short coronin isoform, coronin A, has been implicated in cytoskeletal reorganization, chemotaxis, phagocytosis and the initiation of multicellular development. Generally thought of as modulators of F-actin, coronin A and its mammalian homologs have also been shown to mediate cellular processes in an F-actin-independent manner. Therefore, it remains unclear whether or not coronin A carries out its functions through its capacity to interact with F-actin. Moreover, the interacting partners of coronin A are not known. Here, we analyzed the interactome of coronin A as well as its interaction with F-actin within cells and in vitro. Inter

SUBMITTER: Fabrice TN 

PROVIDER: S-EPMC7073074 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature