Combining rare alleles and grouped pollen donors to assign paternity in pollen dispersal studies.

ABSTRACT:

Premise

Pollen dispersal plays a critical role in gene flow of seed plants. Most often, pollen dispersal is measured using paternity assignment. However, this approach can be time-consuming because it typically entails genotyping all pollen donors, receptors, and offspring at several molecular markers.

Methods

We developed a faster, simpler protocol to track paternity, using pollen receptors and grouped pollen donors that possess rare alleles. We tested this approach using wind-pollinated Amaranthus tuberculatus and insect-pollinated Solanum lycopersicum. After screening potential markers for rare alleles, we grew both species in experimental arrays under field conditions.

Results

All tested A. tuberculatus seeds and 97% of S. lyc

SUBMITTER: Butcher CL 

PROVIDER: S-EPMC7073328 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature