Isoform-resolved correlation analysis between mRNA abundance regulation and protein level degradation.

ABSTRACT: Profiling of biological relationships between different molecular layers dissects regulatory mechanisms that ultimately determine cellular function. To thoroughly assess the role of protein post-translational turnover, we devised a strategy combining pulse stable isotope-labeled amino acids in cells (pSILAC), data-independent acquisition mass spectrometry (DIA-MS), and a novel data analysis framework that resolves protein degradation rate on the level of mRNA alternative splicing isoforms and isoform groups. We demonstrated our approach by the genome-wide correlation analysis between mRNA amounts and protein degradation across different strains of HeLa cells that harbor a high grade of gene dosage variation. The dataset revealed that specific biological processes, cellular organelles, spat

SUBMITTER: Salovska B 

PROVIDER: S-EPMC7073818 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature