Effects of Castration on miRNA, lncRNA, and mRNA Profiles in Mice Thymus.

ABSTRACT: Thymic degeneration and regeneration are regulated by estrogen and androgen. Recent studies have found that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are involved in organ development. In this study, RNA sequencing (RNA-seq) results showed that ovariectomy significantly affected 333 lncRNAs, 51 miRNAs, and 144 mRNAs levels (p < 0.05 and |log2fold change| > 1), and orchiectomy significantly affected 165 lncRNAs, 165 miRNAs, and 208 mRNA levels in the thymus. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differentially expressed genes (DEGs) were closely related to cell development and immunity. Next, we constructed two lncRNA-miRNA-mRNA networks using Cytoscape based on the targeting relationship between differentially expressed miRNA

SUBMITTER: Li B 

PROVIDER: S-EPMC7074395 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature