Self-Redirection of Metabolic Flux Toward Squalene and Ethanol Pathways by Engineered Yeast.

ABSTRACT: We have previously reported that squalene overproducing yeast self-downregulate the expression of the ethanol pathway (non-essential pathway) to divert the metabolic flux to the squalene pathway. In this study, the effect of co-production of squalene and ethanol on other non-essential pathways (fusel alcohol pathway, FA) of Saccharomyces cerevisiae was evaluated. However, before that, 13 constitutive promoters, like IRA1p, PET9p, RHO1p, CMD1p, ATP16p, USA3p,RER2p, COQ1p, RIM1p, GRS1p,MAK5p, and BRN1p, were engineered using transcription factor bindings sites from strong promoters HHF2p (-300 to -669 bp) and TEF1p (-300 to -579 bp), and employed to co-overexpress squalene and ethanol pathways in S. cerevisiae. The FSE strain overexpressing the key genes of the squalene pathway accumulated 5

SUBMITTER: Manzoor R 

PROVIDER: S-EPMC7074498 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature