On-chip MIC by Combining Concentration Gradient Generator and Flanged Chamber Arrays.

ABSTRACT: Minimum inhibition concentration (MIC) of antibiotic is an effective value to ascertain the agent and minimum dosage of inhibiting bacterial growth. However, current techniques to determine MIC are labor intensive and time-consuming, and require skilled operator and high initial concentration of bacteria. To simplify the operation and reduce the time of inhibition test, we developed a microfluidic system, containing a concentration generator and sub-micro-liter chambers, for rapid bacterial growth and inhibition test. To improve the mixing effect, a micropillar array in honeycomb-structure channels is designed, so the steady concentration gradient of amoxicillin can be generated. The flanged chambers are used to culture bacteria under the condition of continuous flow and the medium of cham

SUBMITTER: Zhang XY 

PROVIDER: S-EPMC7074598 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature