Nanoscale Phenotypic Textures of Yersinia pestis Across Environmentally-Relevant Matrices.

ABSTRACT: The persistence of bacterial pathogens within environmental matrices plays an important role in the epidemiology of diseases, as well as impacts biosurveillance strategies. However, the adaptation potentials, mechanisms for survival, and ecological interactions of pathogenic bacteria such as Yersinia pestis are largely uncharacterized owing to the difficulty of profiling their phenotypic signatures. In this report, we describe studies on Y. pestis organisms cultured within soil matrices, which are among the most important reservoirs for their propagation. Morphological (nanoscale) and phenotypic analysis are presented at the single cell level conducted using Atomic Force Microscopy (AFM), coupled with biochemical profiles of bulk populations using Fatty Acid Methyl Ester Profiling (FAME).

SUBMITTER: Iqbal KM 

PROVIDER: S-EPMC7074701 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature