The histone methyltransferase Setd8 alters the chromatin landscape and regulates the expression of key transcription factors during erythroid differentiation.

ABSTRACT:

Background

SETD8 is the sole methyltransferase capable of mono-methylating histone H4, lysine 20. SETD8 and H4K20me1 play a role in a number of essential biologic processes, including cell cycle progression, establishment of higher order chromatin structure, and transcriptional regulation. SETD8 is highly expressed in erythroid cells and erythroid deletion of Setd8 is embryonic lethal by embryonic day 11.5 (E11.5) due to profound anemia, suggesting that it has an erythroid-specific function. The function of SETD8 in the hemopoietic system is poorly understood. The goal of our study was to gain insights into the function of SETD8 during erythroid differentiation.

Results

We performed ATAC-seq (assay for transposase-accessible chromatin) on sorted populations of E10.5 Setd8 mu

SUBMITTER: Myers JA 

PROVIDER: S-EPMC7075014 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature