Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H₂O₂ addition.

ABSTRACT:

Background

Biological α-olefins can be used as both biofuels and high value-added chemical precursors to lubricants, polymers, and detergents. The prototypic CYP152 peroxygenase family member OleT_JE from Jeotgalicoccus sp. ATCC 8456 catalyzes a single-step decarboxylation of free fatty acids (FFAs) to form α-olefins using H₂O₂ as a cofactor, thus attracting much attention since its discovery. To improve the productivity of α-olefins, significant efforts on protein engineering, electron donor engineering, and metabolic engineering of OleT_JE have been made. However, little success has been achieved in obtaining α-olefin high-producer microorganisms due to multiple reasons such as the tight regulation of FFA biosynthesis, the difficul

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC7075034 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature