STRsearch: a new pipeline for targeted profiling of short tandem repeats in massively parallel sequencing data.

ABSTRACT:

Background

Short tandem repeats (STRs) are important polymorphism makers for human identification and kinship analyses in forensic science. With the continuous development of massively parallel sequencing (MPS), more laboratories have utilized this technology for forensic applications. Existing STR genotyping tools, mostly developed for whole-genome sequencing data, are not effective for MPS data. More importantly, their backward compatibility with the conventional capillary electrophoresis (CE) technology has not been evaluated and guaranteed.

Results

In this study, we developed a new end-to-end pipeline called STRsearch for STR-MPS data analysis. The STRsearch can not only determine the allele by counting repeat patterns and INDELs that are actually in the STR region, but

SUBMITTER: Wang D 

PROVIDER: S-EPMC7075041 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature