Dynamic insights on transcription initiation and RNA processing during bacterial adaptation.

ABSTRACT: Transcription initiation and RNA processing govern gene expression and enable bacterial adaptation by reshaping the RNA landscape. The aim of this study was to simultaneously observe these two fundamental processes in a transcriptome responding to an environmental signal. A controlled σ^E system in E. coli was coupled to our previously described tagRNA-seq method to yield process kinetics information. Changes in transcription initiation frequencies (TIF) and RNA processing frequencies (PF) were followed using 5' RNA tags. Changes in TIF showed a binary increased/decreased pattern that alternated between transcriptionally activated and repressed promoters, providing the bacterial population with transcriptional oscillation. PF variation fell into three categories of cleavag

SUBMITTER: Lacoux C 

PROVIDER: S-EPMC7075262 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature