Ontology highlight
ABSTRACT:
SUBMITTER: Dantuluri S
PROVIDER: S-EPMC7075268 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Dantuluri Swathi S Abdullahu Leonora L Munir Annum A Katolik Adam A Damha Masad J MJ Shuman Stewart S
RNA (New York, N.Y.) 20200113 4
The enzyme Tpt1 removes an internal RNA 2'-PO<sub>4</sub> via a two-step reaction in which: (i) the 2'-PO<sub>4</sub> attacks NAD<sup>+</sup> to form an RNA-2'-phospho-(ADP-ribose) intermediate and nicotinamide; and (ii) transesterification of the ADP-ribose O2″ to the RNA 2'-phosphodiester yields 2'-OH RNA and ADP-ribose-1″,2″-cyclic phosphate. Because step 2 is much faster than step 1, the ADP-ribosylated RNA intermediate is virtually undetectable under normal circumstances. Here, by testing c ...[more]