Multiplexed profiling of RNA and protein expression signatures in individual cells using flow or mass cytometry.

ABSTRACT: Advances in single-cell analysis technologies are providing novel insights into phenotypic and functional heterogeneity within seemingly identical cell populations. RNA within single cells can be analyzed using unbiased sequencing protocols or through more targeted approaches using in situ hybridization (ISH). The proximity ligation assay for RNA (PLAYR) approach is a sensitive and high-throughput technique that relies on in situ and proximal ligation to measure at least 27 specific RNAs by flow or mass cytometry. We provide detailed instructions for combining this technique with antibody-based detection of surface/internal protein, allowing simultaneous highly multiplexed profiling of RNA and protein expression at single-cell resolution. PLAYR overcomes limitations on multiplexing seen in

SUBMITTER: Duckworth AD 

PROVIDER: S-EPMC7075472 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature