Preclinical evaluation of [¹⁸F]FB-A20FMDV2 as a selective marker for measuring α_Vβ₆ integrin occupancy using positron emission tomography in rodent lung.

ABSTRACT:

Purpose

Integrin α_vβ₆ belongs to the RGD subset of the integrin family, and its expression levels are a prognostic and theranostic factor in some types of cancer and pulmonary fibrosis. This paper describes the GMP radiolabelling of the synthetic 20 amino acid peptide A20FMDV2 (NAVPNLRGDLQVLAQKVART), derived from the foot-and-mouth disease virus, and characterises the use of [¹⁸F]FB-A20FMDV2 as a high affinity, specific and selective PET radioligand for the quantitation and visualisation of α_vβ₆ in rodent lung to support human translational studies.

Methods

The synthesis of [¹⁸F]FB-A20FMDV2 was performed using a fully automated and GMP-compliant process. Sprague-Dawley rats were used to perform homologous (unl

SUBMITTER: Onega M 

PROVIDER: S-EPMC7075836 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature