Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing.

ABSTRACT: Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing. In addition, there is not yet a robust way of controlling spheroid size, homogeneity and integrity during extended culture. This work addresses these bottlenecks to the automation of hepatocyte spheroid culture by tethering 3D hepatocyte spheroids directly onto surface-modified polystyrene (PS) multi-well plates. However, polystyrene surfaces are inert toward functionalization, and this makes the uniform conjugation of bioactive ligands very challenging. Surface modification

SUBMITTER: Tasnim F 

PROVIDER: S-EPMC7075904 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature