Role of plasmid carrying bla _NDM in mediating antibiotic resistance among Acinetobacter baumannii clinical isolates from Egypt.

ABSTRACT: We investigated antibiotic resistance levels among bla _NDM -positive (n = 9) and -negative (n = 65) A. baumannii clinical isolates collected in 2010 and 2015 from Alexandria Main University Hospital, Egypt using disc diffusion and minimum inhibitory concentration (MIC) determination. Plasmids from bla _NDM -positive isolates were transformed into a carbapenem-susceptible A. baumannii (CS-AB) isolate to assess the role of plasmid transfer in mediating carbapenem resistance. Imipenem, meropenem, and ertapenem MIC90 values against bla _NDM -positive isolates were 128, > 256, and 256 µg/mL, respectively. Plasmid isolation and polymerase chain reaction revealed that bla _NDM

SUBMITTER: Abouelfetouh A 

PROVIDER: S-EPMC7076099 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature