Development of an In Vitro System to Study the Interactions of Aerosolized Drugs with Pulmonary Mucus.

ABSTRACT: Mucus is the first biological component inhaled drugs encounter on their journey towards their pharmacological target in the upper airways. Yet, how mucus may influence drug disposition and efficacy in the lungs has been essentially overlooked. In this study, a simple in vitro system was developed to investigate the factors promoting drug interactions with airway mucus in physiologically relevant conditions. Thin layers of porcine tracheal mucus were prepared in Transwell® inserts and initially, the diffusion of various fluorescent dyes across those layers was monitored over time. A deposition system featuring a MicroSprayer® aerosolizer was optimized to reproducibly deliver liquid aerosols to multiple air-facing layers and then exploited to compare the impact of airway mucus on the transp

SUBMITTER: Alqahtani S 

PROVIDER: S-EPMC7076363 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature