Mitochondrial DNA Repair in an Arabidopsis thaliana Uracil N-Glycosylase Mutant.

ABSTRACT: Substitution rates in plant mitochondrial genes are extremely low, indicating strong selective pressure as well as efficient repair. Plant mitochondria possess base excision repair pathways; however, many repair pathways such as nucleotide excision repair and mismatch repair appear to be absent. In the absence of these pathways, many DNA lesions must be repaired by a different mechanism. To test the hypothesis that double-strand break repair (DSBR) is that mechanism, we maintained independent self-crossing lineages of plants deficient in uracil-N-glycosylase (UNG) for 11 generations to determine the repair outcomes when that pathway is missing. Surprisingly, no single nucleotide polymorphisms (SNPs) were fixed in any line in generation 11. The pattern of heteroplasmic SNPs was also unalter

SUBMITTER: Wynn E 

PROVIDER: S-EPMC7076443 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature