A PCR, qPCR, and LAMP Toolkit for the Detection of the Wheat Blast Pathogen in Seeds.

ABSTRACT: Wheat blast is a devastating disease caused by the pathogenic fungus Pyricularia oryzae. Wheat blast first emerged in South America before more recently reaching Bangladesh. Even though the pathogen can spread locally by air-dispersed spores, long-distance spread is likely to occur via infected wheat seed or grain. Wheat blast epidemics are caused by a genetic lineage of the fungus, called the Triticum lineage, only differing from the other P. oryzae lineages by less than 1% genetic divergence. In order to prevent further spread of this pathogen to other wheat-growing areas in the world, sensitive and specific detection tools are needed to test for contamination of traded seed lots by the P. oryzaeTriticum lineage. In this study, we adopted a comparative genomics approach to identify new l

SUBMITTER: Thierry M 

PROVIDER: S-EPMC7076445 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature