Inhibiting Iron Mobilization from Bacterioferritin in Pseudomonas aeruginosa Impairs Biofilm Formation Irrespective of Environmental Iron Availability.

ABSTRACT: Although iron is essential for bacteria, the nutrient presents problems of toxicity and solubility. Bacteria circumvent these problems with the aid of iron storage proteins where Fe³⁺ is deposited and, when necessary, mobilized as Fe²⁺ for metabolic requirements. In Pseudomonas aeruginosa, Fe³⁺ is compartmentalized in bacterioferritin (BfrB), and its mobilization as Fe²⁺ requires specific binding of a ferredoxin (Bfd) to reduce the stored Fe³⁺. Blocking the BfrB-Bfd complex leads to irreversible iron accumulation in BfrB and cytosolic iron deprivation. Consequently, given the intracellular iron sufficiency requirement for biofilm development, we hypothesized that blocking the BfrB-Bfd interaction in P. aeruginosa would im

SUBMITTER: Soldano A 

PROVIDER: S-EPMC7076691 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature