Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach.

ABSTRACT:

Background

Blood-retinal barrier cells are known to exhibit a massive phenotypic change during experimental autoimmune uveitis (EAU) development. In an attempt to investigate the mechanisms of blood-retinal barrier (BRB) breakdown at a global level, we studied the gene regulation of total retinal cells and retinal endothelial cells during non-infectious uveitis.

Methods

Retinal endothelial cells were isolated by flow cytometry either in Tie2-GFP mice (CD31⁺ CD45^- GFP⁺ cells), or in wild type C57BL/6 mice (CD31⁺ CD45^- endoglin⁺ cells). EAU was induced in C57BL/6 mice by adoptive transfer of IRBP1-20-specific T cells. Total retinal cells and retinal endothelial cells from naïve and EAU mice were sorted and their

SUBMITTER: Lipski DA 

PROVIDER: S-EPMC7076950 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature