Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life.

ABSTRACT:

Background

Leucine-rich repeats and immunoglobulin-like domains 1 (Lrig1) regulates stem cell quiescence. As a marker, it identifies stem cells in multiple organs of the mouse. We had detected Lrig1 expression in cultured Id1^high neural stem cells obtained from the lateral walls lining the lateral ventricles of the adult mouse brain. Thus, we investigated whether Lrig1 expression also identifies stem cells in that region in vivo.

Methods

Publicly available single cell RNA sequencing datasets were analyzed with Seurat and Monocle. The Lrig1+ cells were lineage traced in vivo with a novel non-disruptive co-translational Lrig1^T2A-iCreERT2 reporter mouse line.

Results

Analysis of single cell RNA sequencing datasets suggested Lrig1 was highly expresse

SUBMITTER: Nam HS 

PROVIDER: S-EPMC7077007 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature