Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice.

ABSTRACT: BACKGROUND:Myrin®-p Forte is an anti-tuberclosis agent that can cause hepatic injuries in clinical settings. Maytenus royleanus (Celastraceae) is a medicinal plant, possesses antioxidant and anticancer activities. The hepatoprotective effect of the methanol extract of Maytenus royleanus leaves (MEM) against Myrin®-p Forte induced hepatotoxicity in mice was investigated. METHODS:Mice were randomly parted into six groups (n?=?6). Fixed-dose combination of Myrin®-p Forte (13.5?mg/kg Rifampicin, 6.75?mg/kg Isoniazid, 36.0?mg/kg Pyrazinamide and 24.8?mg/kg Ethambutol; RIPE] was administered for 15?days to induce liver injury. In treatment groups MEM (200?mg/kg and 400?mg/kg doses) and Vitamin B6 (180mg/kg) were administered prior to RIPE. Control group received 2% DMSO. Serum liver function tests, DNA damage, tissue antioxidant enzymes and histopathological alterations were studied. HPLC analysis was performed to determine the chemical composition using standard compounds. RESULTS:The quercitin, gallic acid, luteolin, viteixin, apigenin, kaempherol, hyperoside and myricetin contents of all samples were determined by reverse-phase HPLC. Quercetin (0.217?mg/g dry weight) and luteolin (0.141?mg/g dry weight) were the major flavonoids identified in MEM. Myrin®-p Forte markedly (p?

SUBMITTER: Shabbir M 

PROVIDER: S-EPMC7077109 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature