Nanoscale Structure Determination of Murray Valley Encephalitis and Powassan Virus Non-Coding RNAs.

ABSTRACT: Viral infections are responsible for numerous deaths worldwide. Flaviviruses, which contain RNA as their genetic material, are one of the most pathogenic families of viruses. There is an increasing amount of evidence suggesting that their 5' and 3' non-coding terminal regions are critical for their survival. Information on their structural features is essential to gain detailed insights into their functions and interactions with host proteins. In this study, the 5' and 3' terminal regions of Murray Valley encephalitis virus and Powassan virus were examined using biophysical and computational modeling methods. First, we used size exclusion chromatography and analytical ultracentrifuge methods to investigate the purity of in-vitro transcribed RNAs. Next, we employed small-angle X-ray

SUBMITTER: Mrozowich T 

PROVIDER: S-EPMC7077200 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature