Quality Assessment of Virus-Like Particles at Single Particle Level: A Comparative Study.

ABSTRACT: Virus-like particles (VLPs) have emerged as a powerful scaffold for antigen presentation and delivery strategies. Compared to single protein-based therapeutics, quality assessment requires a higher degree of refinement due to the structure of VLPs and their similar properties to extracellular vesicles (EVs). Advances in the field of nanotechnology with single particle and high-resolution analysis techniques provide appealing approaches to VLP characterization. In this study, six different biophysical methods have been assessed for the characterization of HIV-1-based VLPs produced in mammalian and insect cell platforms. Sample preparation and equipment set-up were optimized for the six strategies evaluated. Electron Microscopy (EM) disclosed the presence of several types of EVs within VLP p

SUBMITTER: Gonzalez-Dominguez I 

PROVIDER: S-EPMC7077327 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature