LXR Activation Induces a Proinflammatory Trained Innate Immunity-Phenotype in Human Monocytes.

ABSTRACT:

Objectives

The concept of trained innate immunity describes a long-term proinflammatory memory in innate immune cells. Trained innate immunity is regulated through reprogramming of cellular metabolic pathways including cholesterol and fatty acid synthesis. Here, we have analyzed the role of Liver X Receptor (LXR), a key regulator of cholesterol and fatty acid homeostasis, in trained innate immunity.

Methods and results

Human monocytes were isolated and incubated with different stimuli for 24 h, including LXR agonists, antagonists and Bacillus Calmette-Guerin (BCG) vaccine. After 5 days resting time, cells were restimulated with the TLR2-agonist Pam3cys. LXR activation did not only increase BCG trained immunity, but also induced a long-term inflammatory activation by itself.

SUBMITTER: Sohrabi Y 

PROVIDER: S-EPMC7077358 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature