Quantitative Analysis of in Vivo Methionine Oxidation of the Human Proteome.

ABSTRACT: The oxidation of methionine is an important post-translational modification of proteins with numerous roles in physiology and pathology. However, the quantitative analysis of methionine oxidation on a proteome-wide scale has been hampered by technical limitations. Methionine is readily oxidized in vitro during sample preparation and analysis. In addition, there is a lack of enrichment protocols for peptides that contain an oxidized methionine residue, making the accurate quantification of methionine oxidation difficult to achieve on a global scale. Herein, we report a methodology to circumvent these issues by isotopically labeling unoxidized methionines with ¹⁸O-labeled hydrogen peroxide and quantifying the relative ratios of ¹⁸O- and ¹⁶O-oxidized methionin

SUBMITTER: Bettinger JQ 

PROVIDER: S-EPMC7077757 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature