Microtubules Increase Diastolic Stiffness in Failing Human Cardiomyocytes and Myocardium.

ABSTRACT:

Background

Diastolic dysfunction is a prevalent and therapeutically intractable feature of heart failure (HF). Increasing ventricular compliance can improve diastolic performance, but the viscoelastic forces that resist diastolic filling and become elevated in human HF are poorly defined. Having recently identified posttranslationally detyrosinated microtubules as a source of viscoelasticity in cardiomyocytes, we sought to test whether microtubules contribute meaningful viscoelastic resistance to diastolic stretch in human myocardium.

Methods

Experiments were conducted in isolated human cardiomyocytes and trabeculae. First, slow and rapid (diastolic) stretch was applied to intact cardiomyocytes from nonfailing and HF hearts and viscoelasticity was characterized after interve

SUBMITTER: Caporizzo MA 

PROVIDER: S-EPMC7078018 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature