Analysis of urinary sphingolipids using liquid chromatography-tandem mass spectrometry in diabetic nephropathy.

ABSTRACT:

Aims/introduction

Sphingolipids, such as ceramides and sphingosine, are involved in the pathogenesis of diabetes; however, the modulation of urinary sphingolipids in diabetic nephropathy has not been fully elucidated. Therefore, we aimed to develop a simultaneous measurement system for urinary sphingolipids using liquid chromatography-tandem mass spectrometry and to elucidate the modulation of urinary sphingolipids in diabetic nephropathy.

Materials and methods

We established a simultaneous measurement system for the urinary sphingosine, dihydrosphingosine, and six ceramide species (Cer d18:1/16:0, Cer d18:1/18:0, Cer d18:1/18:1, Cer d18:1/20:0, Cer d18:1/22:0 and Cer d18:1/24:0), and we examined the urinary sphingolipids in 64 type 2 diabetes patients and 15 control partici

SUBMITTER: Morita Y 

PROVIDER: S-EPMC7078086 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature