Wingless-type MMTV integration site family member 5a is a key inhibitor of islet stellate cells activation.

ABSTRACT:

Aims/introduction

Type 2 diabetes mellitus is a chronic metabolic disorder characterized by islet β-cell dysfunction, which might result from the activation of islet stellate cells (ISCs). Our recent study showed that a specific population of ISCs is prone to be activated in type 2 diabetes mellitus accompanied by reduced secretion of insulin. The wingless-type MMTV integration site family member 5a (Wnt5a)/frizzled-5 signaling pathway might play an important role in this process. The present study aimed to explore the effects of Wnt5a on the activation of ISCs isolated from db/db mice.

Materials and methods

ISCs were isolated from db/db mice and matched db/m mice. Immunohistochemistry and western blotting analysis were applied for the determination of Wnt5a expression. Exog

SUBMITTER: Xu W 

PROVIDER: S-EPMC7078096 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature