Visualization of unstained homo/heterogeneous DNA nanostructures by low-voltage scanning transmission electron microscopy.

ABSTRACT: Three-dimensional (3D) homo/heterogeneous DNA nanostructures were studied with low-voltage scanning transmission electron microscopy (LV-STEM). Four types of 3D DNA nanostructures were designed and fabricated by the origami method including newly proposed protocols. The low-energy electron probe and optimized dark-field STEM detector enabled individual unstained DNA nanostructures to be clearly imaged by the single acquisition without the averaging process. For the vertically stacked double structures, assembled through modified single-stranded domains, and the structures containing a square opening (i.e., a hole) in the center, the LV-STEM successfully reveals the vertical information of these 3D structures as the contrast differences compared to the reference. For the heterogeneous struc

SUBMITTER: Gang GW 

PROVIDER: S-EPMC7078320 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature