Extended hypothermic oxygenated machine perfusion enables ex situ preservation of porcine livers for up to 24 hours.

ABSTRACT:

Background & aims

End-ischemic hypothermic oxygenated machine perfusion (HOPE) of the donor liver for 1-2 h mitigates ischemia-reperfusion injury during subsequent liver transplantation. Extended preservation time may be preferred to facilitate difficult recipient hepatectomy or to optimize logistics. We therefore investigated whether end-ischemic dual HOPE (DHOPE) could extend preservation time for up to 24 h using a porcine liver reperfusion model.

Methods

Following 30 min warm ischemia, porcine livers were subjected to 2 h static cold storage (SCS), followed by 2 h, 6 h, or 24 h DHOPE (n = 6 per group). Subsequent normothermic reperfusion was performed for 4 h using autologous blood. Two livers preserved by 24 h SCS served as additional controls. A proof of principle conf

SUBMITTER: Bruggenwirth IMA 

PROVIDER: S-EPMC7078381 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature