Kruppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells.

ABSTRACT:

Background & aims

Self-renewal and multipotent differentiation are cardinal properties of intestinal stem cells (ISCs), mediated in part by WNT and NOTCH signaling. Although these pathways are well characterized, the molecular mechanisms that control the 'stemness' of ISCs are still not well defined. Here, we investigated the role of Krüppel-like factor 5 (KLF5) in regulating ISC functions.

Methods

We performed studies in adult Lgr5^{EGFP-IRES-creERT2};Rosa26^LSLtdTomato (Lgr5^Ctrl) and Lgr5^{EGFP-IRES-creERT2};Klf5^fl/fl;Rosa26^LSLtdTomato (Lgr5^ΔKlf5) mice. Mice were injected with tamoxifen to activate Cre recombinase, which deletes Klf5 from the intestinal epithelium in Lgr5^ΔKlf5 but not Lgr5

SUBMITTER: Kim CK 

PROVIDER: S-EPMC7078555 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature