Diffusion Mapping of Eosinophil-Activation State.

ABSTRACT: Eosinophils are granular leukocytes that play a role in mediating inflammatory responses linked to infection and allergic disease. Their activation during an immune response triggers spatial reorganization and eventual cargo release from intracellular granules. Understanding this process is important in diagnosing eosinophilic disorders and in assessing treatment efficacy; however, current protocols are limited to simply quantifying the number of eosinophils within a blood sample. Given that high optical absorption and scattering by the granular structure of these cells lead to marked image features, the physical changes that occur during activation should be trackable using image analysis. Here, we present a study in which imaging flow cytometry is used to quantify eosinophil activation s

SUBMITTER: Piasecka J 

PROVIDER: S-EPMC7079009 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature