Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.

ABSTRACT:

Background & aims

The extrahepatic bile duct is the primary tissue initially affected by biliary atresia. Biliary atresia is a cholangiopathy which exclusively affects neonates. Current animal models suggest that the developing bile duct is uniquely susceptible to damage. In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.

Methods

We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse. The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.

Results

SUBMITTER: Khandekar G 

PROVIDER: S-EPMC7079197 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature