Joint single cell DNA-seq and RNA-seq of gastric cancer cell lines reveals rules of in vitro evolution.

ABSTRACT: Cancer cell lines are not homogeneous nor are they static in their genetic state and biological properties. Genetic, transcriptional and phenotypic diversity within cell lines contributes to the lack of experimental reproducibility frequently observed in tissue-culture-based studies. While cancer cell line heterogeneity has been generally recognized, there are no studies which quantify the number of clones that coexist within cell lines and their distinguishing characteristics. We used a single-cell DNA sequencing approach to characterize the cellular diversity within nine gastric cancer cell lines and integrated this information with single-cell RNA sequencing. Overall, we sequenced the genomes of 8824 cells, identifying between 2 and 12 clones per cell line. Using the transcriptomes of m

SUBMITTER: Andor N 

PROVIDER: S-EPMC7079336 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature