Crosstalk between DNA methylation and histone acetylation triggers GDNF high transcription in glioblastoma cells.

ABSTRACT:

Background

Glial cell line-derived neurotrophic factor (GDNF) is highly expressed in glioblastoma (GBM) and blocking its expression can inhibit the initiation and development of GBM. GDNF is a dual promoter gene, and the promoter II with two enhancers and two silencers plays a major role in transcription initiation. We had previously reported that histone hyperacetylation and DNA hypermethylation in GDNF promoter II region result in high transcription of GDNF in GBM cells, but the mechanism remains unclear. In this study, we investigated whether these modifications synergistically regulate high GDNF transcription in GBM.

Results

Cyclic AMP response element binding protein (CREB) expression and phosphorylation at S133 were significantly increased in human GBM tissues and GBM

SUBMITTER: Zhang B 

PROVIDER: S-EPMC7079383 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature