Generating linear oxygen gradients across 3D cell cultures with block-layered oxygen controlled chips (BLOCCs).

ABSTRACT: Oxygen is a transcriptional regulator responsible for tissue homeostasis and maintenance. Studies relating cellular phenotype with oxygen tension often use hypoxia chambers, which expose cells to a single, static oxygen tension. Despite their ease of use, these chambers are unable to replicate the oxygen gradients found in healthy and diseased tissues. Microfabricated devices capable of imposing an oxygen gradient across tissue-like structures are a promising tool for these studies, as they can provide a high density of information in a single experimental setup. We describe the fabrication and characterization of a modular device, which leverages the gas-permeability of silicone to impose gradients of oxygen across cell-containing regions, assembled by layering sheets of laser cut acrylic

SUBMITTER: Boyce MW 

PROVIDER: S-EPMC7079814 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature