Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach.

ABSTRACT: Elucidating how the spatial organization of extrinsic signals modulates cell behavior and drives biological processes remains largely unexplored because of challenges in controlling spatial patterning of multiple microenvironmental cues in vitro. Here, we describe a high-throughput method that directs simultaneous assembly of multiple cell types and solid-phase ligands across length scales within minutes. Our method involves lithographically defining hierarchical patterns of unique DNA oligonucleotides to which complementary strands, attached to cells and ligands-of-interest, hybridize. Highlighting our method's power, we investigated how the spatial presentation of self-renewal ligand fibroblast growth factor-2 (FGF-2) and differentiation signal ephrin-B2 instruct single adult neural stem

SUBMITTER: Scheideler OJ 

PROVIDER: S-EPMC7080440 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature