A single-cell transcriptome atlas for zebrafish development.

ABSTRACT: The ability to define cell types and how they change during organogenesis is central to our understanding of animal development and human disease. Despite the crucial nature of this knowledge, we have yet to fully characterize all distinct cell types and the gene expression differences that generate cell types during development. To address this knowledge gap, we produced an atlas using single-cell RNA-sequencing methods to investigate gene expression from the pharyngula to early larval stages in developing zebrafish. Our single-cell transcriptome atlas encompasses transcriptional profiles from 44,102 ​cells across four days of development using duplicate experiments that confirmed high reproducibility. We annotated 220 identified clusters and highlighted several strategies for interrogati

SUBMITTER: Farnsworth DR 

PROVIDER: S-EPMC7080588 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature