New methods for objective angiogenesis evaluation of rat nerves using microcomputed tomography scanning and conventional photography.

ABSTRACT:

Introduction

Nerve regeneration involves multiple processes, which enhance blood supply that can be promoted by growth factors. Currently, tools are lacking to visualize the vascularization patterns in transplanted nerves in vivo. The purpose of this study was to describe three-dimensional visualization of the vascular system in the rat sciatic nerve and to quantify angiogenesis of nerve reconstruction.

Materials and methods

In 12 Lewis rats (weighing 250-300 g), 10 mm sciatic nerve gaps were repaired with ipsilateral reversed autologous nerve grafts. At 12 and 16 weeks of sacrifice, Microfil® contrast compound was injected in the aorta. Nerve autografts (N = 12) and contralateral untreated nerves (N = 12) were harvested and cleared while preserving the vasculature. The amou

SUBMITTER: Saffari TM 

PROVIDER: S-EPMC7080612 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature