Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water.

ABSTRACT: Signal enhancements of up to two orders of magnitude in protein NMR can be achieved by employing HDO as a vector to introduce hyperpolarization into folded or intrinsically disordered proteins. In this approach, hyperpolarized HDO produced by dissolution-dynamic nuclear polarization (D-DNP) is mixed with a protein solution waiting in a high-field NMR spectrometer, whereupon amide proton exchange and nuclear Overhauser effects (NOE) transfer hyperpolarization to the protein and enable acquisition of a signal-enhanced high-resolution spectrum. To date, the use of this strategy has been limited to 1D and ¹H-¹⁵N 2D correlation experiments. Here we introduce 2D ¹³C-detected D-DNP, to reduce exchange-induced broadening and other relaxation penalties that can adve

SUBMITTER: Olsen GL 

PROVIDER: S-EPMC7080779 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature