High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells.

ABSTRACT:

Significance

Photobiomodulation is a well-established therapeutic modality. However, the mechanism of action is poorly understood, due to lack of research in the causal relationship between the near-infrared (NIR) light irradiation and its specific biological effects, hindering broader applications of this technology.

Aim

Since biological chromophores typically show several absorption peaks, we determined whether specific effects of photobiomodulation are induced with a combination of two wavelengths at a certain range of irradiance only, rather than a single wavelength of NIR light.

Approach

In order to analyze a wide array of combinations of multispectral NIR light at various irradiances efficiently, we developed a new optical platform equipped with two distinct wav

SUBMITTER: Katagiri W 

PROVIDER: S-EPMC7081057 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature