Migratory dendritic cells in skin-draining lymph nodes have nickel-binding capabilities.

ABSTRACT: Nickel (Ni) is the most frequent metal allergen and induces Th1-dependent type-IV allergies. In local skin, epidermal Langerhans cells (LCs) and/or dermal dendritic cells (DCs) uptake antigens and migrate to draining lymph nodes (LNs). However, the subsets of antigen-presenting cells that contribute to Ni presentation have not yet been identified. In this study, we analyzed the Ni-binding capabilities of murine DCs using fluorescent metal indicator Newport Green. Elicitation of Ni allergy was assessed after intradermal (i.d.) injection of Ni-treated DCs into ear pinnae of Ni-sensitized mice. The Ni-binding capabilities of MHC class II^hi CD11c^int migratory DCs were significantly stronger than those of MHC class II^int CD11c^hi resident DCs and CD11c

SUBMITTER: Kuroishi T 

PROVIDER: S-EPMC7081353 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature