Capsid-Labelled HIV To Investigate the Role of Capsid during Nuclear Import and Integration.

ABSTRACT: The HIV-1 capsid protein performs multiple roles in virus replication both during assembly and particle release and during virus trafficking into the nucleus. In order to decipher the roles of capsid protein during early replication, a reliable method to follow its intracellular distribution is required. To complement existing approaches to track HIV-1 capsid during early infection, we developed an HIV-1 imaging strategy, relying on viruses incorporating enhanced green fluorescent protein (eGFP)-tagged capsid (CA-eGFP) protein and mCherry-tagged integrase (IN-mCherry). Wild-type infectivity and sensitivity to inhibition by PF74 point to the functionality of CA-eGFP-containing complexes. Low numbers of CA-eGFP molecules were located inside the viral core and imported into the nucleus withou

SUBMITTER: Zurnic Bonisch I 

PROVIDER: S-EPMC7081887 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature