Cupredoxin engineered upconversion nanoparticles for ratiometric luminescence sensing of Cu².

ABSTRACT: The NIR excitation and large anti-Stokes shift of upconversion nanoparticles (UCNPs) have made them an ideal choice as biological nanoprobes. A key challenge in the field is to confer biorecognition units to UCNPs so that they can be used to probe specific targets in biological systems. While various agents have been combined with UCNPs to meet such a challenge, most studies are limited to small molecules, while biomolecules such as metalloproteins that possess much higher affinity and selectivity for metal ions have not been explored. Herein we demonstrate that fusion of zwitterion-coated UCNPs with azurin, a member of a family of redox-active copper proteins called cupredoxins that play important roles in diverse biological functions, can serve as an ideal platform for the label-free upc

SUBMITTER: Liu C 

PROVIDER: S-EPMC7081933 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature